C22-107-08 rHuCNTF說明書

Catalog Number:  C22-107-08
Source:  Escherichia coli.
Molecular Weight:  Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
Quantity:  5μg/20μg/1000μg
AA Sequence:  MAFTEHSPLT PHRRDLCSRS IWLARKLRSD LTALTESYVK HQGLNKNINL DSADGMPVAS
 TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA
 YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS
 SHQTGIPARG SHYIANNKKM
Purity:  >97% by SDS-PAGE and HPLC analyses.
Biological Activity:  Fully biologically active when compared to standard. The ED50 determined by a cell proliferation
 assay using human TF-1 cells is less than 2 ng/ml, corresponding to a specific activity of＞ 5.0 × 105 IU/mg.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin:  Less than 1EU/μg of rHuCNTF as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Human Ciliary Neurotrophic Factor
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic
factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a
survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons,
embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the
degeneration of motor axons after axotomy.
The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human
CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and
exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is