C22-102-08 rHuPEDF說明書

Catalog Number:  C22-102-08
Source:  Escherichia coli.
Molecular Weight:  Approximately 44.5 KDa, a single non-glycosylated polypeptide chain containing 400 amino acids.
Quantity:  5μg/20μg/1000μg
AA Sequence:  MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV SNFGYDLYRV RSSMSPTTNV
 LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS
 ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE
 IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD
 LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK
 LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ
 PAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP
Purity:  >95% by SDS-PAGE and HPLC analyses.
Biological Activity:  Data Not Available.
Physical Appearance:  Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:  Lyophilized from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4, 150mM NaCl.
Endotoxin:  Less than 1EU/μg of rHuPEDF as determined by LAL method.
Reconstitution:  We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the
 bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a
 concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and
 stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage:  This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage,
 preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For
 maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to
 -70°C. Avoid repeated freeze/thaw cycles.
Usage:  This material is offered by Shanghai Corning Bio-Tech for research, laboratory or further
 evaluation purposes. NOT FOR HUMAN USE.

Human Pigment Epithelium-derived Factor
Pigment Epithelium-derived Factor (PEDF) is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic
properties. It is a 50 kDa glycoprotein produced and secreted in many tissues throughout the body. A major component of the
anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit
the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through
suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. The recent identification of a lipaselinked
cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of
the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a
serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic agent for the treatment of
vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a
number of cancers.