人分化因子-5（原骨形態發生蛋白- 14）|Human GDF-5 (Formerly BMP-14)

Synonyms :

BMP-14, Cartilage-Derived Morphogenetic Protein-1 (CDMP-1)

Description :

GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 354 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease. Recombinant human GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids. License: This product is produced solely by PeproTech Inc. under a license from Biopharm GmbH, Germany. Biopharm GmbH is not responsible for the quality or fitness for use of this product. It may be used only for research purposes and may neither be used in humans nor for veterinary purposes. This product may not be used for commercial purposes.

Catalog #:

120-01

Source :

E.coli

Stability :

The lyophilized protein is stable for at least 2 years from date of receipt at -20⁰C. Reconstituted GDF-5 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20⁰C. Avoid repeated freeze/thaw cycles.

Purity :

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin Level :

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Biological Activity :

The ED₅₀ was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1.0-2.0 µg/ml.

AA Sequence :

APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR

PubMed Link :

CDMP-1+OR+GDF-5+OR+BMP-14

Country of Origin :

USA