產品資料

細胞解離產品

產品名稱: 細胞解離產品
產品型號: C14-13150-016
產品展商: GIBCO
產品文檔: 無相關文檔

簡單介紹

細胞解離液Cell dissociation buffer 不含酶,由Hank’s液配制 保存:15-30°


細胞解離產品  的詳細介紹
詳細說明:
Cell Dissociation Buffers are membrane-filtered, isotonic, and enzyme-free aqueous formulations of salts, chelating agents, and cell-conditioning agents in either Ca2+- and Mg2+-free Hanks balanced salt solution or Ca2+- and Mg2+-free phosphate-buffered saline. They are suitable for gentle dissociation of mammalian cells from support substrates and each other, for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry (1,2). These buffers are not recommended for routine passage of strongly adherent cell types.

Performance and quality testing: Buffers are membrane filtered and mycoplasma tested. Cell integrity after dissociation with buffers is measured with a plating efficiency assay and a serotonin receptor function assay.

常用規格

Chelators:

EDTA 

Product Size:

100 ml 

Classification:

Animal Origin-Free 

Enzyme Stability:

Room Temperature Stable 

Phenol Red Indicator:

No Phenol Red 


相關信息:

品名

產品描述

貨號

規格

細胞解離液

Cell dissociation buffer

不含酶,由Hank’s液配制

保存:15-30°

13150-016

100ml

細胞解離液

Cell dissociation buffer

不含酶,由PBS配制

保存:15-30°

13151-014

100ml

膠原酶,collagenase

活性:每毫克不少于125U,2-8°

17018-029

500mg

I型膠原酶,collagenase Type I

可解離組織,建議用于上皮、肺、脂肪和腎上腺組織細胞,2-8°保存

17100-017

1g

II型膠原酶,collagenase Type II

可解離組織,建議用于肝、骨、甲狀腺、心和唾液腺組織細胞,2-8°保存

17101-015

1g

IV型膠原酶,collagenase Type IV

可解離胰腺組織,用于分離胰島,

2-8°保存

17104-019

1g

分散酶,Dispase

溫和的解離組織得到單個細胞,

2-8°保存

17105-041

5g

胰酶替代物,TrypLE Select

配方包含1mM EDTADPBS,

不含酚紅,保存:2-8°

12563-011

100ml

 

12563-029

500ml

胰酶替代物,TrypLE Express

配方包含1mM DPBS,

含酚紅,保存:2-8°

12605-010

100ml

 

12605-028

500ml

胰酶替代物,TrypLE Express

配方包含1mMDPBS,

不含酚紅,保存:2-8°

12604-013

100ml

 

12604-021

500ml

胰酶,Trpsin,0.25%

不含鈣鎂離子,含酚紅

保存:-5 - -20°

15050-065

100ml

 

15050-057

500ml

胰酶,Trpsin,2.5%10X

無菌條件下以Hank’s10倍稀釋,

不含鈣鎂離子,保存:-5 - -20°

15090-046

100ml

胰酶-EDTA,

0.05%胰酶,EDTA。4Na

不含鈣鎂離子,含酚紅

保存:-5 - -20°

25300-054

100ml

25300-062

500ml

胰酶-EDTA,(10X

0. 5%胰酶,EDTA。4Na

無菌條件下以Hank’s10倍稀釋,

不含鈣鎂離子,保存:-5 - -20°

15400-054

100ml

胰酶-EDTA,

0.25%胰酶,EDTA。4Na

不含鈣鎂離子,含酚紅

保存:-5 - -20°

25200-056

100ml

25200-072

500ml

胰酶(1250),干粉

保存:2-8°

27250-018

100g

胰酶抑制劑,大豆

0.25-0.5mg/mlPBS溶液滅活等體積胰酶,保存:-5 - -20°

17075-029

1g

EDTA,Versene,15000

0.2g/l EDTA.4Na磷酸緩沖鹽溶液,

=0.48Mm,保存:2-8°

15040-066

100ml

Low Trypsin-High EDTA,

0.025% 胰酶, 0.75mM EDTA (1X), 溶于PBS,不含鈣鎂離子

SM-2004-C

100ml

Trypsin 0.25%

Hank‘s 液,不含鈣鎂離子

SM-2001-C

100ml

Accutase

 

SCR005

100ml

Accumax

 

SCR006

100ml

Trypsin-EDTA

0.05% Trypsin & 0.53mM EDTA,

溶于Hank‘s液,不含鈣鎂

SM-2002-C

100ml

Trypsin-EDTA

0.25% Trypsin & 1mM EDTA,

溶于Hank‘s液,不含鈣鎂離子

SM-2003-C

100ml




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