加拿大戴爾豪斯醫學院癌癥研究人員帕特里克﹒李博士證明，一種普通的病毒可以感染并殺死癌癥干細胞。這一突破性發現發表在*近出版的美國基因療法學會雜志《分子療法》上。
科學界近幾年才剛認識到癌癥干細胞的作用，并迫切需要找到一種**它的辦法?！鞍┌Y干細胞是一種母細胞?！崩畈┦拷忉屨f?！八鼈儾粩喈a生新的癌細胞并形成腫瘤?！庇捎诎┌Y干細胞對化療和放療不敏感，因此要殺死它們非常困難。就像李博士所說，“我們可以殺死腫瘤里的所有常規癌細胞，但只要存在癌癥干細胞，癌癥就會復發?！?p style="padding-right: 0px; padding-left: 0px; font-size: 1.2em; padding-bottom: 0px; margin: 0px 0px 12px; line-height: 1.8em; padding-top: 0px">“我們認為呼腸孤病毒能有效殺死癌癥干細胞，因為我們反復發現它能有效殺死常規癌細胞?！崩畈┦空f。他是世界上**位發現一種良性自然病毒能有選擇感染并殺死癌細胞而不損害健康細胞的人。加拿大一家生物技術公司對呼腸孤病毒進行臨床試驗發現，這種****而有效。
與大多數利用試驗室培養癌細胞的研究不同，這次研究所用的是從患者身上切除的新鮮乳腺癌組織。
呼腸孤病毒除了具有殺死癌細胞和癌癥干細胞的能力外，還能激發**的**系統。由于這種**也能誘導**反應，李博士和同事正在研究一種方法，在病毒感染并摧毀癌癥細胞的同時能加強**系統抗擊癌細胞的能力?！拔覀兿乱徊焦ぷ骶褪亲屵@種雙重**的方法能夠成熟?！崩畈┦空f?！拔覀円煤裟c孤病毒固有的特點和**系統自身優勢開發一種強有力的、基于病毒的**療法?！?p style="padding-right: 0px; padding-left: 0px; font-size: 1.2em; padding-bottom: 0px; margin: 0px 0px 12px; line-height: 1.8em; padding-top: 0px">呼腸孤病毒有效針對癌癥干細胞這一研究發現已引起英國LeadDiscovery制藥公司的注意，他們準備進行這一方面的**研發。（創賽新聞中心canspecsci.com）
創賽推薦原始出處：
Molecular Therapy (2009); doi:10.1038/mt.2009.58
Oncolytic Reovirus Effectively Targets Breast Cancer Stem Cells
Paola Marcato1, Cheryl A Dean1, Carman A Giacomantonio2 and Patrick WK Lee1,3
1Department of Microbiology and Immunology, Faculty ofMedicine,Dalhousie University, Halifax, Nova Scotia, Canada
2Department of Surgery, Faculty of Medicine, DalhousieUniversity,Halifax, Nova Scotia, Canada
3Department of Pathology, Faculty of Medicine, DalhousieUniversity,Halifax, Nova Scotia, Canada
Abstract
Recent evidence suggests that cancer stem cells (CSCs) play animportant role incancer, as these cells possess enhancedtumor-forming capabilitiesand are resistant to current anticancertherapies. Hence, novelcancer therapies will need to be tested forboth tumor regressionand CSC targeting. Herein we show thatoncolytic reovirus thatinduces regression of human breast cancerprimary tumor samples xenografted in immunocompromised mice also effectively targets and kills CSCs in these tumors. CSCs were identified based on CD24-CD44+ cell surface expression and overexpression of aldehyde dehydrogenase. Upon reovirus treatment, the CSC population was reduced at the same rate as non-CSCs within the tumor. Immunofluorescence of breast tumor tissue samples from the reovirus- and mock-treated mice confirmed that both CSCs and non-CSCs were infectible by reovirus, and terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) assay showed that both populations died by apoptosis. Ras, which has been shown to mediate reovirus oncolysis, was found to be present at similar levels in all cell types, and this is consistent with their comparable sensitivity to reovirus. These experiments indicate that oncolytic reovirus has the potential to induce tumor regression in breast cancer patients. More important, the CSC population was equally reduced and was as susceptible to reovirus treatment as the non-CSC population.
